Detailed studies on the embryotoxic and teratogenic effects of synthetic cannabinoids known to be abused are very limited. The present study aimed to evalutate the possible embryotoxic, teratogenic, behavioral, and molecular effects of 4F-MDMB-BUTICA, a new generation synthetic cannabinoid, using zebrafish embryos. The zebrafish embryos were exposed to the cannabinoid at 0.15, 0.30, 0.60, 1.20, 2.40, and 4.80 mg/L from 3 to 24 hpf (acute) and 3 to 120 hpf (subacute). No developmental abnormalities and mortality were observed in embryos after acute exposure. Subacute 4F-MDMB-BUTICA exposure induced mortality of the embryos with the 120 hpf LC