Detection of Oral Submucous Fibrosis at the Crossroads of Altering Nucleogeometry, Nuclear Spatial Distribution and Nuclear Molecular Abundance.

• Tác giả: Sourangshu Chakraborti, Jyotirmoy Chatterjee, Raunak Kumar Das, Mousumi Pal, Ranjan Rashmi Paul
• Ngôn ngữ: eng
• Thông tin xuất bản: India : Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India , 2025
• Bộ sưu tập: NCBI
• ID: 697625

Tóm tắt

The manuscript concentrates on spatial distribution and various nucleomorphometric parameters of epithelium to diagnose Oral Submucous Fibrosis (OSF). Histologically confirmed OSF and normal submucosa tissue samples were procured and stained with diamidino phenylindole (DAPI) to visualize nuclei. E-cadherins and p63 were also immunohistochemically stained. Microphotographs were analyzed to quantify the spatial distance among the nuclei of the stained tissue samples. Intensity of the DAPI stained nuclei and p63 was quantified. In addition, morphometric analysis of nuclei was done with the help of