Single-molecule tracking in living microbial cells.

• Tác giả: Xiaomin Chen, Jun Fan, Jiexin Guan, Qianhong Guo, Ting Jiang, Liping Xie, Lu Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 785.13 *Trios
• Thông tin xuất bản: China : Biophysics reports , 2025
• Bộ sưu tập: NCBI
• ID: 697800

Tóm tắt

Some microbes are referred to as model organisms because they are easy to study in the laboratory and hold the ability to retain their characteristics during DNA replication, DNA transcription, and other fundamental processes. Studying these microbes in living cells via single-molecule imaging allows us to better understand these processes at highly improved spatiotemporal resolution. Single particle tracking photoactivated localization microscopy (sptPALM) is a robust tool for detecting the positions and motions of individual molecules with tens of nanometers of spatial and millisecond temporal resolution