High-Throughput Proteoform Imaging for Revealing Spatial-Resolved Changes in Brain Tissues Associated with Alzheimer's Disease.

• Tác giả: Yu Liang, Zhen Liang, Dan Liu, Xinxin Liu, Yue Sun, Xue Yang, Lihua Zhang, Yukui Zhang, Baofeng Zhao
• Ngôn ngữ: eng
• Thông tin xuất bản: Germany : Advanced science (Weinheim, Baden-Wurttemberg, Germany) , 2025
• Bộ sưu tập: NCBI
• ID: 698689

Tóm tắt

Spatially resolved characterization of proteoforms has substantial potential to significantly advance the understanding of physiological and disease mechanisms. However, challenges remain regarding throughput and coverage. A robust method is developed for high-throughput proteoform imaging (HTPi) by combining matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) and region-specific top-down proteomic analysis. MALDI MSI enables the imaging of proteoforms on tissue sections at a rate of 7 h cm