BACKGROUND/OBJECTIVES: Intestinal alkaline phosphatase (IAP) is an enzyme expressed in the intestinal brush border, which may exert anti-inflammatory effects by detoxifying lipopolysaccharides (LPSs), thereby preventing metabolic disorders. Various food components have been reported to influence IAP activity. However, few studies have evaluated the effects of fermented milk on IAP activity. In this study, we aimed to investigate fermented milk with high IAP-activating capacity and investigate its effect. METHODS: We screened a skim milk culture (SC), a fermented milk model, using differentiated Caco-2 cells. We investigated the effect of SC on IAP activity and gene expression in the RESULTS: Among the SC samples from different lactic acid bacteria or bifidobacteria, the SC of CONCLUSIONS: LJ0309 SC increased IAP activity and gene expression in both Caco-2 cells and the