Spheroids Composed of Reaggregated Neonatal Porcine Islets and Human Endothelial Cells Accelerate Development of Normoglycemia in Diabetic Mice.

• Tác giả: Kevin Bellofatto, Ekaterine Berishvili, Francesco Campo, Antonio Citro, Alessia Cucci, Antonia Follenzi, Mohsen Honarpisheh, Elisabeth Kemter, Martin Kraetzl, Fanny Lebreton, Yutian Lei, Cristina Olgasi, Lorenzo Piemonti, Cataldo Pignatelli, Jochen Seissler, Olivier Thaunat, Vanguard Consortium, Eckhard Wolf, Lelia Wolf-van Buerck
• Ngôn ngữ: eng
• Thông tin xuất bản: Switzerland : Cells , 2025
• Bộ sưu tập: NCBI
• ID: 698972

Tóm tắt

The engraftment of transplanted islets depends on the rapid establishment of a novel vascular network. The present study evaluated the effects of cord blood-derived blood outgrowth endothelial cells (BOECs) on the viability of neonatal porcine islets (NPIs) and the post-transplant outcome of grafted NPIs. Dispersed NPIs and human BOECs were reaggregated on microwell cell culture plates and tested for their anti-apoptotic and pro-angiogenic capacity by qRT-PCR and immunohistochemistry. The in vivo functionality was analyzed after transplantation into diabetic NOD-SCID IL2rγ