1. This study examined feeding practices that could affect the expression of intestinal calcium transporter gene, tibial mass, eggshell quality and production performance in 25-week-old Hy-Line Brown Laying Hens.2. In this study, 360 healthy 25-week-old Hy-Line Brown laying hens were divided into three treatment groups with 10 replicates of 12 hens per group. The groups were fed as follows: 1) Twice a day, 50% of the standard breeder diet was fed at 8:00 and 15:00 (CFG)
2) Two-thirds of the standard breeder diet was fed at 8:00 and 15:00 (UFG), respectively
3) Two different diets were fed separately, 50% at 8:00 and 50% at 15:00 (SFG)3. The feed-to-egg ratios of the CFG and SFG groups were significantly higher than those of the UFG group. The eggshell weight of the UFG group was numerically reduced (