This study aimed to assess the biological activities of Tunisian Simmondsia chinensis and characterize its potential bioactive compounds. Different extracts of S. chinensis were tested for their antioxidant, antibacterial, anti-α-amylase, and anti-acetylcholinesterase activities through in vitro assays. The methanolic extract exhibited the highest levels of antioxidant activity and total phenolic content (976.03 GAE/g extract) compared to the other extracts. Additionally, it demonstrated a substantial anti-acetylcholinesterase activity (PI=75 %) and potent antibacterial property, particularly against Enterococcus faecalis, Listeria monocytogenes, Bacillus cereus, Bacillus subtilis, and Salmonella enterica. The IC