The influence of peripheral antitussive drugs on spatiotemporal features of coughing has not been reported. We hypothesized that this class of compounds would alter the cough motor pattern, in part, by lengthening cough phases. Peripherally acting antitussives, 3-aminopropylphosphinic acid (3APPi, 5 mg/kg) and levodropropizine (Levo, 3 mg/kg) were injected intravenously in anesthetized spontaneously breathing cats (13 males, 2 females
4.38 ± 0.19 kg). Spatio-temporal analysis of cough induced by mechanical stimulation of the trachea showed significant reductions in cough number and expiratory cough efforts after the administration of each drug. A significant reduction in inspiratory cough efforts occurred after Levo. Both drugs induced temporal changes in the cough motor pattern, including prolongations of inspiratory phase, inspiratory-expiratory transition, total cough diaphragm activity, and total cough cycle duration. Levo also significantly lengthened the expiratory phase of cough. A shortening of the overlap between diaphragm and abdominal activity and cough abdominal electromyogram (EMG) activity was observed after the administration of 3APPi. No significant changes in cardiorespiratory data were seen, with the exception of prolonged expiratory phase after 3APPi and lower blood pressure after Levo. Peripherally induced cough suppression is accompanied with changes in cough temporal characteristics that are not observed after the administration of centrally acting antitussives. The motor output produced by the cough central pattern generator differs significantly when coughing is perturbed by peripherally and centrally acting antitussives.