Using ultrasound technology as one of the therapeutic methods, in which ultrasound waves of different frequencies and intensities are employed, has significantly contributed to enhancing and facilitating the treatment process of various diseases. A Hemostatic Ultrasonic Scalpel can entail considerable advantages by simultaneously performing two operations tissue cutting and coagulation of biological tissues. In the present study, employing experimental design through response surface methodology, the effect of ultrasonic power and the duration of vibration application on the tissue has been investigated. Two parameters, namely the burst pressure of the sealed vessel and the length of the thermal seal zone, were measured by pressure testing and analysis image of the thermal effect region at the sealed vessel area, respectively. The pressure test results demonstrated that an input power of 52 W and the application of vibrations for 8 s under a constant force of 10 N, showed the optimized maximum burst pressure equal to 1100 mmHg. Examination of the sealed vessel images revealed a linear increase in thermal damage with increasing input power.