Synthetic chitobiose-containing glycolipid (GL) and lipid (L) are prepared in order to secure self-assembled multivalent glycostructures, constituted with varying molar fractions of GL and L. The morphologies of glycostructures are uniform, as adjudged by dynamic light scattering (DLS) in solution and microscopies in the solid state. Presence of the ester linkage between the lipid and chitobiose moieties permit hydrolysis and disassembly of the self-assembled structures at acidic and alkaline pH. The avidity of chitobiose in the multivalent glycostructures to lysozyme follows the percentage of GL in the GL-L compositions in the order 50 % GL >
100 % GL-L >
10 % GL-L. The interaction with lysozyme occurs with fast association and slow dissociation kinetics, from which the equilibrium binding constant (K