Preferential superficial cortical layer activation during seizure propagation.

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Tác giả: Anastasia Brodovskaya, Jaideep Kapur, Edward Perez-Reyes, Shinnosuke Shiono, Chengsan Sun

Ngôn ngữ: eng

Ký hiệu phân loại: 629.292 Nonsurface motor land vehicles

Thông tin xuất bản: United States : Epilepsia , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 700600

OBJECTIVE: Focal cortical seizures travel long distances from the onset zone, but the long-distance propagation pathways are uncertain. In vitro and in vivo imaging techniques have investigated the local spread of seizures but did not elucidate long-distance spread. Furthermore, classical studies in slices suggested seizure spread locally along deep cortical layers, whereas more recent in vivo imaging studies posit a role for superficial cortical layers in local spread. METHODS: We imaged seizure-activated neurons using activity reporter mice and measured local field potentials (LFPs) using microelectrode arrays to map cortical seizure propagation in awake mice. RESULTS: Frontal lobe onset seizures activate more neurons in superficial layers 2-3 than deep layers 5-6 throughout the cortex. LFP recordings demonstrate that seizures spread faster through the superficial than deep layers over long cortical distances of 3.5 mm. We also show that monosynaptically connected long-distance neurons are in the seizure circuit. SIGNIFICANCE: We propose that long-distance cortical seizure spread occurs preferentially via synaptically connected superficial cortical neurons.
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