Clothianidin (CTD), a highly water soluble neonicotinoid insecticide, easily enters water through runoff. Developing eco-friendly materials to degrade CTD is essential. Nano zero valent iron (nZVI) is effective for contaminant removal, but it deactivates due to agglomeration. Biochar supported sulfidated nano zero valent iron (S-nZVI-BC) can effectively mitigate nZVI aggregation while enhancing anti-passivation and electron transfer. However, the regulation of BC preparation conditions on S-nZVI-BC performance and contaminant degradation mechanism remains elusive. This work systematically investigated the effects of BC pyrolysis temperature on FeS formation in S-nZVI-BC and CTD degradation mechanism. BC enhanced FeS crystallinity and increased Fe