Aggregation processes of microalgae have significant effects on the vertical distribution of microplastics (MPs) in the marine environment. This study explored how the harmful microalga Heterosigma akashiwo affects the aggregation and sinking characteristics of four types of MPs: low and high-density polyethylene (PE) spheres, and small and large polypropylene (PP) fragments. The aggregation of MPs was primarily driven by extracellular polymeric substances (EPS) rather than direct attachment to the cells, contributing to their sinking. The sinking of low-density PE spheres followed a logistic function, saturating at 28 % with a half-saturation time of 9 days. In contrast, small PP fragments sank minimally (under 2 %) and large PP fragments showed almost no sinking, indicating the varying impacts of MP density and size. The sinking velocity of the MP aggregates was significantly lower for low-density PE spheres (0.63 mm∙s