Molecular and functional changes in GABAergic transmission during epileptogenesis in a rat model of post-traumatic epilepsy.

• Tác giả: Pierangelo Cifelli, Angela Di Iacovo, Meinrad Drexel, Alessandro Gaeta, Anssi Lipponen, Xavier Ekolle Ndode-Ekane, Eleonora Palma, Asla Pitkänen, Noora Puhakka, Cristina Roseti, Gabriele Ruffolo, Günther Sperk, Johanna Tiilikainen
• Ngôn ngữ: eng
• Ký hiệu phân loại: 523.019 Molecular, atomic, nuclear physics
• Thông tin xuất bản: United States : Experimental neurology , 2025
• Bộ sưu tập: NCBI
• ID: 701893

Tóm tắt

Traumatic brain injury (TBI) is one of the leading causes of structural epilepsy. Our objective was to investigate the molecular and functional dysregulation of GABAergic neurotransmission during a wide time window from acute to chronic phases of epileptogenesis after TBI. Perilesional and thalamic tissues sampled from a clinically relevant animal model of post-traumatic epilepsy induced by lateral fluid-percussion injury were investigated using in situ hybridization, immunohistochemistry and RNA sequencing. For functional analysis, we utilized a membrane microtransplantation technique in Xenopus oocytes in order to overcome the technical difficulties that would stem from recording directly from highly damaged lesional and perilesional brain tissues. Already at 6 to 24 h post-TBI we found a dysregulation in the expression of GABA