Based on previous research results, iron-dependent autotrophic denitrification (IDAD) was evaluated in an all-around way to provide a theoretical basis for further research. First, this review systematically and comprehensively summarizes the development of IDAD technology and describes the physiological properties of relevant functional microorganisms and their potential mechanisms from different perspectives. Second, the possible Fe-N pathways involved in the reaction of different iron-based materials are discussed in detail. Then, the theoretical advantages of the IDAD process and potential problems are described, and the corresponding control strategies are summarized. The influence of key factors on denitrification is discussed in terms of operational and water quality parameters. In addition, the application and research direction of this technology in engineering are summarized. Finally, the latest development trends and prospects for future applications are discussed to promote an in-depth understanding of IDAD and its practical application in sewage treatment.