Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness.

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Tác giả: Roman Belousov, Anna Erzberger, Takashi Hiiragi, Takafumi Ichikawa, Chizuru Iwatani, Prachiti Moghe, Tomoyuki Tsukiyama

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Nature cell biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 702478

Tissue patterning coordinates morphogenesis, cell dynamics and fate specification. Understanding how precision in patterning is robustly achieved despite inherent developmental variability during mammalian embryogenesis remains a challenge. Here, based on cell dynamics quantification and simulation, we show how salt-and-pepper epiblast and primitive endoderm (PrE) cells pattern the inner cell mass of mouse blastocysts. Coupling cell fate and dynamics, PrE cells form apical polarity-dependent actin protrusions required for RAC1-dependent migration towards the surface of the fluid cavity, where PrE cells are trapped due to decreased tension. Concomitantly, PrE cells deposit an extracellular matrix gradient, presumably breaking the tissue-level symmetry and collectively guiding their own migration. Tissue size perturbations of mouse embryos and their comparison with monkey and human blastocysts further demonstrate that the fixed proportion of PrE/epiblast cells is optimal with respect to embryo size and tissue geometry and, despite variability, ensures patterning robustness during early mammalian development.
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