A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis.

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Tác giả: Masafumi Hidaka, Yuji Honda, Kazuhiko Ishikawa, Takane Katayama, Toshihiko Katoh, Takashi Koyanagi, Hidehiko Kumagai, Chiaki Matsuzaki, Yukari Nakashima

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Japan : Proceedings of the Japan Academy. Series B, Physical and biological sciences , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 702589

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Enhancing the thermostability of cellulose-degrading enzymes is pivotal for establishing an efficient bioconversion system from cellulosic materials to value-added compounds. Here, by introducing random and saturation mutagenesis into the Thermoascus aurantiacus β-glucosidase gene, we generated a hyperthermostable mutant with five amino acid substitutions. Analysis of temperature-induced unfolding revealed the involvement of each replacement in the increased T

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