The whitefly, Bemisia tabaci, is among the most important threats to global agriculture and food security. In addition to losses associated with feeding, B. tabaci vectors hundreds of plant viruses, many of which cause severe disease in staple food crops. The management of B. tabaci is confounded by extensive resistance to chemical insecticides. While pesticidal proteins derived from entomopathogenic bacteria such as Bacillus thuringiensis (Bt) could provide for alternative management approaches, only one pesticidal protein with toxicity to B. tabaci has been identified. Here we screened 11 Bt-derived pesticidal proteins from several different structural classes against the highly invasive, Middle East-Asian Minor 1 (MEAM1) cryptic species of B. tabaci, and assessed the impact of a B. tabaci-active protein on the gut epithelial membrane by transmission electron microscopy. The pesticidal proteins were expressed in Bt or in Escherichia coli and purified for use in bioassays. The toxicity of purified proteins was first assessed by feeding adults on a single dose followed by lethal concentration (LC