Electrochemical nitrite sensing (ENS) is a competitive method for online monitoring in the intelligent control of biological nitrogen removal process. However, its popularity is extremely low due to complex wastewater interference and low sensor durability. Here, we developed a novel ENS method that utilizes the mass transfer signal (MTS) of the nitrite oxidation reaction (NOR), making detection accuracy dependent solely on mass transfer process. These features enabled us to design a catalyst-free, small-sized glassy carbon rotating disk electrode for accurate MTS determination with exceptional durability. The linearity of MTS versus nitrite concentration surpasses that of conventional differential pulse voltammetry and amperometry. The method has a wide linear range of 100 μM-100 mM, a detection limit of 28 μM, and a high sensitivity of 1638 μA mM