This paper presents a novel approach for estimating Dissolved Organic Carbon (DOC) concentrations in lakes considering both carbon sources and sink operators. Despite the critical role of DOC, the combined application of machine learning, as a robust predictor, and remote sensing technology, which reduces costly and time-intensive in-situ sampling, has been underexplored in DOC research. Focusing on lakes over the states of New York, Vermont and Maine (United States, U.S.), this study integrates in-situ DOC measurements with surface reflectance bands obtained from Landsat satellites between 2000 and 2020. Using these bands as inputs of the Random Forest (RF) predictive model, the introduced methodology aims to explore the ability of remote sensing data for large-scale DOC simulation. Initial results indicate low accuracy metrics and significant under-estimation due to the imbalance distribution of DOC samples. Statistical analysis showed that the mean DOC concentration was 5.37±3.37 mg/L (mean±one standard deviation), with peak up to 25 mg/L. A highly skewed distribution of chemical components towards the lower ranges can lead to model misrepresentation of extreme and hazardous events, as they are clouded by unimportant events due to significantly lower occurrence rates. To address this issue, the Synthetic Minority Over-sampling Technique (SMOTE) was applied as a key innovation, generating synthetic samples that enhance RF accuracy and reduce the associated errors. Fusion of in-situ and remote sensing data, combined with machine learning and data augmentation, significantly enhances DOC estimation accuracy, especially in high concentration ranges which are critical for environmental health. With prediction metrics of RMSE = 1.75, MAE = 1.09, and R