The impacts of manure dissolved organic matter (DOM) on the adsorption and desorption of veterinary antibiotics in soils have not been fully explored. In this study, the effects of pig manure (PM) DOM and its hydrophilic and hydrophobic fractions on antibiotic adsorption and subsequent desorption in three topsoils of bamboo stands under different fertilization conditions (control, with heavy manure application, and with heavy manure application suspended) were examined by batch experiments, with the aid of excitation-emission matrix fluorescence spectroscopy, Fourier transformed infrared spectroscopy, and X-ray photoelectron spectroscopy. The addition of 100 mg-C L