Begonia fimbristipula Hance (Begoniaceae) is a valuable medicinal herb that is classified as a protected species in Guangdong Province, China. In this study, we present a chromosome-level genome assembly of B. fimbristipula, aiming to facilitate its conservation and utilization. The genome was assembled using a combination of Oxford Nanopore long-read data and Illumina short-read data. The assembled genome size of B. fimbristipula is 462.11 Mb, with a scaffold N50 of 38.22 Mb. A total of 91.96% (424.94 Mb) of the sequences were anchored to 11 pseudochromosomes using Hi-C technology. The genome assembly exhibits a BUSCO completeness of 90.3% and an LTR Assembly Index (LAI) of 17.73. Genome annotation revealed 25,563 protein-coding genes and 274 tRNA genes. The high-quality chromosome-level assembly and annotation provide valuable insights into the genomic characteristics of B. fimbristipula, thereby offering essential resources for its conservation and economic utilization.