This study examined the number of contractions required for an isometric plantar flexion familiarization. Twenty-six males were separated into two independent Groups: Group A: where five contractions were initiated on the dominant limb (right) followed by the contralateral limb
and Group B, initiated by the non-dominant limb (left) followed by the dominant limb. Participants carried out a 5-s maximum voluntary contraction (MVC), with an interpolated twitch administered to the tibial nerve. In Group A, both the dominant and non-dominant limbs required two contractions for familiarization, whereas in Group B, the dominant limb required two contractions, and the non-dominant limb required three (p <
0.05). A strong relationship between MVC and voluntary activation (VA) was observed in Group A for the D (r = 0.91, p <
0.05) and in Group B for the ND limb (r = 0.99, p <
0.05). The results demonstrated evidence of cross-limb transfer from the dominant to the non-dominant limb (p <
0.05). This phenomenon implicates central involvement, substantiated by VA responses that mirror the changes in MVC. Practitioners and researchers should consider the impact of cross-limb transfer during a single familiarization session when assessing strength to avoid overestimation of the gains. Future cross-education/cross-limb transfer studies could investigate the central mechanisms involved during familiarization.