BACKGROUND: Knowledge of Aedes species distribution, preference to feed on humans, and susceptibility to viruses is crucial in preventing transmission of Aedes-transmitted viruses. This study aimed to determine resting behavior, blood sources, and viral infection status of Aedes aegypti in Awash Sebat, Awash Arba, and Werer towns of Afar Region. METHODS: Adult mosquitoes were collected using a Prokopack aspirator between 8:00-14:00 and 15:00-18:00 h both indoor and outdoor of the house. The mosquitoes were sorted by sex, date of collection, collection places, and abdominal status and identified by species/genus using standard keys. Blood meal sources and dengue virus and chikungunya virus infection status of Ae. aegypti were determined using ELISA and RT-qPCR respectively. RESULT: A total of 2,745 adult mosquitoes comprising the genera Aedes (1433
52.2%) Culex (1292
47.1%) and Anopheles (20
0.7%) were collected. The proportion of female Ae. aegypti in Awash Sebat (611
36%) was highest as compared to females Ae. aegypti in Awash Arba (172
33.8%), and in Werer (59
11%). A higher proportion of outdoor resting of Ae. aegypti was caught from tyres rather than other indoor and outdoor locations (314
37.29%) (X CONCLUSION: The majority of Ae. aegypti collections were made during the wet season from outdoor resting sites, particularly from tyres. Thus, outdoor targeted management of Ae. aegypti is recommended as a strategy particularly tyre removal during the wet season, to reduce resting and proliferation of Ae. aegypti and hence prevent the risks of Aedes-borne disease transmission.