UNLABELLED: Disease-suppressive soils have been documented in many economically important crops, but not in turfgrass, one of the most intensively managed plant systems in the United States. Dollar spot, caused by the fungus IMPORTANCE: Given the current reliance on fungicides for plant disease control, this research provides new insights into the potential non-target effects of repeated fungicide usage on disease-suppressive soils. It also indicates that intensive fungicide usage can decrease the activity of beneficial soil microbes and lead to a more disease conducive microbial environment in turfgrass. The results from this study can be used to identify more sustainable disease management strategies for a variety of economically important and intensively managed pathosystems. Understanding the factors that facilitate disease-suppressive soils will contribute to more sustainable plant protection practices.