BACKGROUND/OBJECTIVES: Radiomics has seen substantial growth in medical imaging
however, its potential in optical coherence tomography (OCT) has not been widely explored. We systematically evaluate the repeatability and reproducibility of handcrafted radiomics features (HRFs) from OCT scans of benign nevi and examine the impact of bin width (BW) selection on HRF stability. The effect of using stable features on a radiomics classification model was also assessed. METHODS: In this prospective study, 20 volunteers underwent test-retest OCT imaging of 40 benign nevi, resulting in 80 scans. The repeatability and reproducibility of HRFs extracted from manually delineated regions of interest (ROIs) were assessed using concordance correlation coefficients (CCCs) across BWs ranging from 5 to 50. A unique set of stable HRFs was identified at each BW after removing highly correlated features to eliminate redundancy. These robust features were incorporated into a multiclass radiomics classifier trained to distinguish benign nevi, basal cell carcinoma (BCC), and Bowen's disease. RESULTS: Six stable HRFs were identified across all BWs, with a BW of 25 emerging as the optimal choice, balancing repeatability and the ability to capture meaningful textural details. Additionally, intermediate BWs (20-25) yielded 53 reproducible features. A classifier trained with six stable features achieved a 90% accuracy and AUCs of 0.96 and 0.94 for BCC and Bowen's disease, respectively, compared to a 76% accuracy and AUCs of 0.86 and 0.80 for a conventional feature selection approach. CONCLUSIONS: This study highlights the critical role of BW selection in enhancing HRF stability and provides a methodological framework for optimizing preprocessing in OCT radiomics. By demonstrating the integration of stable HRFs into diagnostic models, we establish OCT radiomics as a promising tool to aid non-invasive diagnosis in dermatology.