The aim of this study was to investigate the impact of incorporating black soldier fly meal (BSFM) into the diet of brooding laying hens, with a focus on growth performance, serum biochemical markers, nutrient digestibility, intestinal morphology, and immune responses. A total of 480 one-day-old chicks (Hy-Line Brown, commercial flock) were randomly divided into four dietary treatment groups, each consisting of six replicates with 20 birds per pen. One group was fed a basal control diet (control/G0 group), while the remaining three groups were fed 3% (G3 group), 6% (G6 group), and 9% (G9 group) BSFM, respectively. The study lasted for 42 days. At both 21 and 42 days of age, several parameters were measured, including average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (FCR), and tibia length. Blood serum samples were also collected to assess biochemical and antioxidant indicators. Results showed that the ADG in the G3 group was significantly higher than the G0 group during the 1-21, 22-24, and 1-42 day periods (