BACKGROUND: To investigate the retinochoroidal and optic nerve head (ONH) microstructural and microvascular age-related changes in healthy subjects by examining the ganglion cell-inner plexiform layer thickness (GC-IPLT), vessel density (VD), and their ratio. METHODS: In this cross-sectional study, 203 subjects (20-69 years old) were divided into five age groups: 20-29 (G1), 30-39 (G2), 40-49 (G3), 50-59 (G4), and 60-69 (G5) (G5). Following a thorough ophthalmological examination, enhanced depth imaging optical coherence tomography (EDI-OCT) scanning was performed along with OCT angiography (OCTA) in a 6 × 6 mm RESULTS: After adjusting for axial length the GC-IPLT varied significantly among groups, with thickness peaking in G3 (39.63 ± 1.14 µm) and then decreasing to the lowest in G5 (34.15 ± 5.93 µm) ( CONCLUSIONS: The retinochoroidal microstructure and microvasculature ratios display an inverted U-shaped pattern with age, which could be linked to a considerably decreased GC-IPLT versus capillary plexus VDs with age, notably in subjects in their sixties.