In modern engineering applications, the use of sustainable materials and eco-friendly methods has become increasingly important. Wood joints, especially those strengthened with bio-adhesive, have attracted considerable attention due to their inherent environmental benefits and desirable mechanical properties. Compared to traditional joining methods, adhesive joints offer unique advantages such as improved load distribution, reduced stress concentration, and enhanced aesthetic appeal. This study aims to enhance delamination resistance in wooden adhesive joints using a novel method involving reinforced high-toughness resin on surfaces. Additionally, a hybrid substrate approach applies a tough layer to outer plies and a densified wood core with greater fiber direction strength. Normal, toughened, and hybrid single-lap joint specimens were analyzed through both experimental and numerical methods under various loading conditions, including quasi-static and intermediate rates. The proposed method involved bio-adhesive penetration into the wood substrate, forming a reinforced surface zone. The experimentally validated results show a significant improvement in joint strength, exhibiting an approximate 2.8-fold increase for the toughened joints compared to the reference joints under intermediate-rate conditions. Furthermore, the absorbed energy of the toughened joints increased by a substantial factor of up to 4.5 times under the same conditions. The fracture surfaces analysis revealed that the toughening method changed the failure mechanism of the joints from delamination to fiber breakage, indicating that the strength of the substrate was lower than that of the joint under impact conditions. The viscoelastic behavior of the bio-adhesive also influenced the response of the joints to the changing displacement rate. The toughening method enhanced the resilience and load-bearing capacity of the wood joints, making them more suitable for dynamic applications.