Detection of Live Shiga Toxin-Producing

• Tác giả: Chin-Yi Chen, Katrina L Counihan, Yiping He, Shannon Tilman
• Ngôn ngữ: eng
• Ký hiệu phân loại: 932.012 Protodynastic, Old Kingdom, first intermediate periods, ca. 3100-2052 B.C.
• Thông tin xuất bản: Switzerland : International journal of molecular sciences , 2025
• Bộ sưu tập: NCBI
• ID: 705672

Tóm tắt

A requirement of any foodborne pathogen testing method is that it only detects live bacteria. Ethidium monoazide (EMA) and propidium monoazide (PMA) are dyes that penetrate the membranes of dead cells and form cross-linkages in the DNA, which prevents its amplification in PCR. This study investigated whether treatment with EMA or PMA would inhibit the sequencing of DNA from dead