Feed additives are crucial in livestock production, enhancing performance, health, and reproductive efficiency. Recently, there has been a shift toward natural biomolecules as feed additives, specifically targeting improved reproductive outcomes and sperm quality. This transition arises from concerns about antibiotic misuse, antimicrobial resistance, and consumer preferences for eco-friendly products, along with the superior bioavailability, lower toxicity, and reduced environmental impact of natural biomolecules compared to synthetic alternatives. Collaboration among researchers, veterinarians, nutritionists, and regulators is essential to ensure safe and effective livestock management. The review explores advancements in using vital biomolecules in reproductive processes, including plant-derived bioactives such as phytochemicals and antioxidants. It investigates not only the mechanisms but also the intricate interactions of these compounds with animals' hormonal and physiological systems. Additionally, the review critically assesses challenges and prospects related to incorporating natural biomolecules into livestock practices. The potential benefits include enhanced reproductive efficiency and improved sperm quality. However, successful implementation requires understanding factors like precise dosing, potential interactions, and long-term health impacts. Overall, this comprehensive review highlights recent research, technological strides, and the future potential of integrating natural biomolecules into animal diets.