Straw-concrete combined floor slabs consist of straw boards, shear-resistant connectors, and concrete slabs. These slabs offer various advantages over traditional reinforced concrete slabs due to the straw boards' properties of excellent insulation and sound absorption. Research using ABAQUS software created 15 composite floor models to study the impact of connection methods, bond strength, connector spacing, and thickness of straw and concrete on the flexural performance. Results indicated that the composite floor slab with adhesive bonding had a 7.34% and 17.34% higher load-carrying capacity than the bolt-connected and self-tapping screw-connected composite floor slabs, respectively. Increasing bond strength from 40 MPa to 60 MPa improved the load-carrying capacity of self-tapping nail-connected slabs by 80.84%. Connector spacing negatively correlated with slab capacity, while increasing the thickness of straw boards or concrete slabs enhanced the ultimate load-carrying capacity, with the latter having a more significant effect. Midspan deflection and flexural capacity were calculated using the converted cross-section method and static calculation formulas, with theoretical and simulated values showing good agreement, offering guidance for engineering applications.