The transition toward sustainable food systems is a critical challenge for the European Union (EU), requiring integrated policies and collaborative action. However, the complexity of food systems and the diverse interests of stakeholders often hinder effective progress. To apply a systemic approach and deliver context-specific solutions, it is necessary to establish key commodity/supply chain groups that include diverse perspectives from multiple stakeholders. Focusing on the Italian wheat supply chain, this research addresses the gap in understanding stakeholder relationships and their influence on the definition and implementation of sustainability measures. It introduces Net-Map analysis as a fit-for-purpose, multistakeholder, participatory approach to map and analyze these relationships, identifying key actors, power dynamics, and opportunities for fostering collaboration. Through a participatory workshop and in-depth interviews, the study gathered data providing insights into stakeholder roles and leverage points. The findings contribute to improved stakeholder involvement in decision-making processes, ultimately supporting the development of a more resilient and sustainable wheat supply chain in Italy.