BACKGROUND: Turkish coffee (TC), a traditional unfiltered coffee preparation method, contains unique bioactive compounds due to its specific brewing process. While TC's cultural significance is well-documented, its acute physiological and psychological effects remain understudied. OBJECTIVES: This randomized, controlled crossover trial investigated the acute effects of a standardized TC dose (3 mg caffeine/kg body weight) on metabolic and psychological parameters in healthy female university students (n = 52, age: 20.25 ± 1.20 years). METHODS: TC was prepared with a 1:1 ratio of medium and dark roasted Arabica beans. The chemical analysis showed the caffeine content to be 2.8 ± 0.3 mg/mL and the chlorogenic acid content to be 1.9 ± 0.2 mg/ml. Participants were randomized to receive either TC or water control, with a washout period of 8 weeks between treatments. Cardiovascular parameters, metabolic markers, and validated visual analog scales (VAS) were assessed at baseline, 60-, 90-, and 120-min post-consumption. RESULTS: Heart rate showed significant time-dependent reductions in both groups (control: CONCLUSIONS: These findings suggest that TC exerts significant acute effects on cardiovascular function and psychophysiological parameters in healthy young females, potentially due to its unique phytochemical profile and preparation method.