This study aimed to describe the ultrasonographic appearance of the liver, spleen, and kidneys in 35 healthy Santa Inês breed sheep. The spleen was located caudal to the last rib and extended to the 8th intercostal space (ICS), while the splenic vein was predominantly visible between the 11th and 10th ICS, located between the 12th and 9th ICS. The liver was visualized from the 12th to the 7th ICS, with the portal vein exhibiting a mean diameter of 15.4 ± 2.0 mm, a rounded shape, and echogenic walls. The caudal vena cava appeared in triangular, elliptical, or round shapes, with a mean diameter of 12.13 ± 3.0 mm. The gallbladder (GB) displayed various shapes, with anechoic to hypoechoic content, and wall measurements of 29.2 ± 11.8 mm in length, 237.4 ± 147.7 mm