OBJECTIVE: The primary aim of this study is to explore the relationships between empathy, organizational support, interprofessional cooperation, burnout, and turnover intention among pharmaceutical professionals working in hospitals and community pharmacies. Additionally, the study seeks to compare the levels of these factors between the two professional groups. DESIGN: An observational and cross-sectional study was conducted. SETTING: Data were collected from community and hospital pharmacies in the Canary Islands. PARTICIPANTS: The sample consisted of 183 pharmaceutical professionals, including 124 working in community settings and 59 in hospital settings. INTERVENTIONS: As an observational study, data collection was carried out without any intervention. MAIN OUTCOME MEASURES: Measures included empathy, interprofessional collaboration and cooperation, perceived organizational support, burnout, and turnover intention. Analyses included correlations, mean comparisons between the hospital and community pharmacy groups, and a regression analysis on turnover intention. RESULTS: The findings revealed that cooperation, coordination, emotional exhaustion, and low personal accomplishment were the factors most strongly associated with turnover intention, explaining 66% of the variance. CONCLUSIONS: The results underscore the importance of these interpersonal factors in promoting occupational well-being among pharmaceutical professionals. The findings are discussed in comparison with previous research conducted in other healthcare professions.