Accurate and timely detection of ions and metabolites in urine is essential for health monitoring. However, traditional methods face challenges in integrating multiplexity, signal stability, and portability. To address these issues, this study developed a 3D-printed portable dual-mode gel microspheres kit. The hydrogel microspheres were prepared by encapsulating the respective indicators: Urine pH Indicator (UpHI) for pH detection, Urine Calcium Ion Indicator (UCaI) for Ca