Human serum albumin (HSA), an important human blood protein, plays a critical role in maintaining osmotic pressure and facilitating the transport of various substances. Abnormal HSA levels are associated with diseases like kidney disease, heart problems, diabetes, and liver damage, necessitating the development of accurate methods for HSA detection. This paper describes the design, synthesis, and evaluation of four BODIPY-based near-infrared (NIR) fluorescent probes (BD1-BD4) for the selective detection of HSA. Among the synthesized probes, BD1 demonstrated exceptional sensitivity and specificity, exhibiting a 147-fold fluorescence enhancement at 660 nm (λ