In this paper, a novel dual-potential ratiometric electrochemiluminescence (RECL) sensor was constructed and applied to the ultrasensitive detection of kanamycin (KAN). The RECL sensor was constructed using porous graphite phase carbon nitride (PCN) and luminol, which serve as the luminescent reagents at the cathode and anode, respectively. PCN not only functioned as the luminescent reagent for the cathode but also served as a carrier for the PtAu NPs. Simultaneously, Ti