Atmospheric cold plasma (ACP), known for its safety, non-thermal processing, and energy efficiency, is especially effective for preserving perishable items such as seafood. However, excessive use of ACP may cause oxidation and sensory quality deterioration, which limits its application in the seafood industry. Thus, optimizing ACP treatment modes to balance sterilization efficiency with minimal oxidative effects is essential. This study aims to explore the impact of different ACP treatment modes (cyclical and one-time treatment) on the muscle quality and bacterial community of red shrimp during cold storage. The results indicated that on day 0 of storage, compared to the blank group and the one-time treatment group, the cyclical treatment significantly reduced the total viable count in red shrimp (p <
0.05). However, no significant differences were observed in centrifugal loss, cooking loss, textural properties, total sulfhydryl content, or Ca