Herein, 12 types of fish oil emulsions were used to prepare 12 types of spray-dried fish oil powders with three gelatins (positive fish gelatin, positive porcine skin gelatin, and negative bovine skin gelatin), two negative pectins (high and low methoxy pectin), and negative sodium starch octenyl succinate (SSOS). The powders consisted of microscale capsules with bi- or monolayer oil/wall interfaces, and monolayer wall layers. SSOS significantly decreased the bulk and tapped densities of the powders. All powders had low water activities (0.14-0.26). Gelatin/pectin and gelatin/pectin/SSOS powders had encapsulation efficiencies of 45.1 %-75.9 % and 70.1 %-93.0 %, respectively. The oxidative stability depended on the compositions of the powder wall layers. FG