Human norovirus (HuNoV) and human sapovirus (HuSaV) are the common foodborne viruses that can be transmitted through the consumption of contaminated shellfish. With the rise of e-commerce, these platforms have become an important channel for Chinese consumers to purchase seafood products. However, the potential impact of this new commercial model on viral contamination necessitates further investigation. This study aimed to compare the levels of HuNoV and HuSaV contamination in oysters (Crassostrea gigas) sourced from physical markets and e-commerce platforms. From July 2021 to June 2022, we collected oysters from both online e-commerce platform (n = 108) and offline markets (n = 108) in Guangzhou to assess HuNoV and HuSaV contamination. The HuNoV contamination rate in oysters from offline markets (61.1%) was found to be higher than that from online e-commerce platforms (54.6%) (p >
0.05). The contamination rate of HuSaV was 16.7% in online e-commerce platforms and 18.5% in offline markets, with a significance level of p >
0.05. Except for the HuNoV GI contamination levels in oysters from offline platforms, which were predominantly in the range of 10