Nghiên cứu tỷ lệ sản sinh men extended-spectrum β-lactamases (ESBL) và phát hiện gen kháng cephlosporin của vi khuẩn Escherichia Coli phân lập từ chất thải lợn tại một số địa phương=Prevalence of extended-spectrum β-lactamases-producing (ESBL) and detection of cephalosporin resistance gene of Escherichia coli isolated from pig manure in Viet Nam

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Tác giả: Thị Thanh Sơn Đặng, Chung Thủy Ngô, Minh Hằng Phạm, Thị Nhật Trần, Xuân Bách Trần, Thị Hương Giang Trương, Thị Quý Dương Trương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2017

Mô tả vật lý:

Bộ sưu tập: Báo, Tạp chí

ID: 70942

Thêm vào giỏ

100 mẫu phân lợn thu thập từ nền chuồng nuôi lợn ở ở ở tỉnh Thái Bình và huyện Sóc Sơn, Hà Nội đã được xét nghiệm nhằm xác định tỷ lệ đa kháng và gen kháng kháng sinh nhóm cephalosporin của các chủng vi khuẩn E. coli. Kết quả nghiên cứu cho thấy E. coli kháng cefotaxime được phát hiện trong 82% số mẫu. Trong tổng số 220 chủng E. coli phân lập được, có 74,1% đa kháng với kháng sinh cephalosporin. Tỷ lệ phát hiện E. coli sản sinh ESBL từ 195 chủng (kháng với ít nhất một loại cephalosporin khác ngoài cefotaxime) là 80,5%. Kết quả nghiên cứu cũng cho thấy 100% các chủng kháng với 5 loại kháng sinh cephalosporin thế hệ 2 và 3 và 97,3% các chủng kháng với 4 loại thế hệ 2 và 3 có khả năng sinh ESBL.Gen CTX và TEM hiện diện phổ biến trong các chủng vi khuẩn E. coli được kiểm tra với tỷ lệ lần lượt là 68,8% và 19,8%. Trong đó, 61,2% số chủng E. coli sinh ESBL chỉ mang gen CTX, 12,1% số chủng chỉ mang gen TEM, và 7,7% số chủng mang đồng thời cả 2 loại gen, không phát hiện được chủng E. coli nào mang gen SHV.This study was conducted to detect the multi-drug resistance E. coli strains and cephalosporin resistance genes of E. coli isolated from 100 pig manure samples that collected from Thai Binh province and Soc Son district, Ha Noi City. The studied result showed that cefotaxime resistant E. coli was found in 82% samples. Among 220 isolated E. coli strains, 74.1% was multi-drug resistance to cephalosporin. The rate of ESBL-producing E. coli strains detecting from 195 strains (resistant to at least with other cephalosporin beside cefotaxime) was 80.5%. Moreover, 100% of 5 antibiotic resistant strains and 97.3% of 4 antibiotic resistant strains were able to produce ESBL.CTX, TEM genes were detected popularly in the tested E. coli strains with the rate was 68.8% and 19.8% respectively, of which 61.2% of the ESBL-producing E. coli strains carried CTX only, 12.1% carried TEM and 7.7% carried both CTX and TEM. The SHV gene was not detected at the current study.

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