BACKGROUND: Pressure injury (PI) is a significant health issue and one of the greatest challenges in critical care units. Guidelines adherence to prevent PIs is not always optimal by critical care nurses. To our knowledge, there is no data about adherence to PI preventive guidelines among Jordanian critical care nurses, and this study aimed to fill this gap. PURPOSE: To assess the levels of adherence, and factors associated with adherence to PIs prevention guidelines among critical care nurses in Jordan. METHODS: A cross-sectional study was conducted among 269 nurses selected from four hospitals in Amman-Jordan. Multiple socio-demographic and psychosocial factors were collected including nurses' knowledge and attitudes toward PI prevention. A valid and reliable self-reported questionnaire (questionnaire to evaluate nurses' adherence to recommendations for preventing pressure ulcers QARPPU) was used to assess participants' adherence to PIs prevention guidelines. Multiple linear regression was conducted to predict the factors associated with adherence to PI prevention guidelines. RESULTS: Nurses reported a mean of 58.2 ± 8.2 (90 is the highest score) of the total score of adherence scale indicating 65 % of adherence to PIs prevention guidelines. Factors independently associated with lower adherence were being a male nurse, absence of a policy of PI prevention, and being a nurse in a non-accredited hospital (all p ≤ 0.05). CONCLUSION: Critical care nurses in Jordan do not have optimal adherence to PIs prevention guidelines. Implementing healthcare policies about PIs prevention and hospital accreditation seems to be essential to enhance their adherence.