This experiment was conducted to investigate the dose effects of iron on growth performance, antioxidant function, small intestinal histology, and intestinal barrier of 63-day-old yellow-feathered broilers. A total of 720 1-day-old male yellow-feathered broilers were randomly divided into 9 treatments, with 8 cages per treatment and 10 birds per cage. The Fe supplementation was 0, 20, 40, 60, 80, 160, 320, 640, and 1280 mg/kg, respectively, in the form of FeSO