Senescent alveolar type II epithelial cells-secreted GDF15 promotes silicosis progression via interfering intercellular communication.

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Tác giả: Demin Cheng, Xinying Jia, Zhenhua Jia, Wenxiu Lian, Yi Liu, Chunhui Ni, Wenqing Sun, Ting Wang

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Netherlands : Ecotoxicology and environmental safety , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 710380

BACKGROUND: Silicosis is a chronic fibrotic pulmonary disease caused by consistent inhalation of respirable crystalline-free silica dust. The senescence of alveolar epithelial type II cells (ATII) is considered the initiation of pulmonary fibrosis. As a secreted protein, growth differentiation factor 15 (GDF15) was found intimately associated with the severity of lung diseases via senescence. Therefore, we speculate that GDF15 may involved in silica-induced pulmonary fibrosis. METHODS: Co-culture was performed to observe the pro-fibrotic effect of GDF15, which is secreted from the silica-induced senescence ATII cells, on peripheral effector cells. We further explored GDF15-related signaling pathways via ChIP and IP assays. GDF15 siRNA lipid nanoparticles, anti-aging compound β-nicotinamide mononucleotide (NMN), and the Chinese traditional drug Bazibushen (BZBS) were used individually to intervene silicosis progress. RESULTS: SiO CONCLUSIONS: Our results elucidate that senescence ATII cell-secreted GDF15 plays a vital role in promoting silicosis by influencing surrounding cells, and provides scientific clues for the selection of potential therapeutic drugs for silicosis.
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