Despite recent surge in microplastics (MPs) research, there is a paucity of information on freshwaters in Ghana. For the first time, MPs in cage and wild sites of the lower Volta Basin were evaluated, and polymer type characterized using LDIR and ATR-FTIR. Seasonal variations and mode of fish production significantly influenced MPs abundance. In fish, MPs concentration of 387 ± 33.85 (wet season) contrasted with 288 ± 21.4 items individual