Chagas disease is a parasitic disease with approximately 8 million people infected worldwide, presenting a limited and toxic treatment. Comprising a vast chemodiversity, microbial metabolites are among the most important sources of FDA-approved anti-infectives. In this work, the bioactivity-guided fractionation from an extract obtained from the bacterium Bacillus altitudinis, isolated from a red seaweed, afforded an antitrypanosomal alkaloid which was characterized as (R)-salsolinol by