Age-related macular degeneration and diabetic retinopathy are the main diseases that cause vision impairment. The standard treatment for this condition is the intravitreal injection of anti-vascular endothelial growth factor agents, which cause several side effects to the eye after injection. Topical administration would be a more effective method, but the ocular layers act as barriers to drug diffusion. In this research, we presented the preparation and characterization of poly(pseudo)rotaxanes (PPRs) containing 2-hydroxypropyl-β-cyclodextrin (2-HPβCD), Pluronic® F127 (PF127) and Soluplus® to enhance the solubility of fenofibrate (FEB), a poorly water-soluble drug, for potential application in ocular drug delivery. The FEB-loaded micelles and PPRs were investigated using DLS,